Replication Study of the Effect of Different Loading Conditions on Running Mechanics at Different Velocities
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Abstract
The aim of this study was to replicate the study titled “Effect of different loading conditions on running mechanics at different velocities” by Carretero-Navarro et al., (2019) as part of a large replication project. The selected variable of interest was leg stiffness. Twenty-six recreationally active and healthy males (age: 23 ± 2 years, body mass: 80.20 ± 11.54kg, height: 177.96 ± 6.29cm) participated in two testing sessions, one week apart. Subjects completed an incremental maximal running test on a treadmill to determine their maximal aerobic speed (MAS). During the second session, participants completed nine, one-minute runs under different loading (+0%, +10%, and +20% of body mass using a weighted vest) and speed (60%, 80%, and 100% of their MAS) conditions. A two-way repeated measures ANOVA showed a significant main effect for speed on leg stiffness (F1.7, 38.6 = 5.94, p = 0.008, ηp2 = 0.205), similar to the original study (F2, 24 = 52.577, p < 0.001). However, the replication effect size estimate for speed on leg stiffness (ηp2 = 0.205) was significantly smaller than the original (ηp2 = 0.814) (z = 4.56, p < 0.001). The original effect size estimate for the main effect of speed was deemed incompatible with the replication estimate, therefore, the original study was not replicated fully. As there are growing demands for enhancing the quality of sports science research, one should focus on the accumulation of evidence for the effect of speed on leg stiffness to maximize athletic performance.
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References
Billat, L.V. and Koralsztein, J.P. (1996) ‘Significance of the velocity at V02max and time to exhaustion at this velocity’, Sports Medicine, 22(1), pp. 90–108. Available at: https://doi.org/10.2165/00007256-199622020-00004.
Blickhan, R. (1989) ‘The spring-mass model for running and hopping’, 22(11), pp. 1217–1227. Available at: https://doi.org/10.1016/0021-9290(89)90224-8.
Brandt, M.J. et al. (2014) ‘The Replication Recipe: What makes for a convincing replication?’, Journal of Experimental Social Psychology, 50(1), pp. 217–224. Available at: https://doi.org/https://doi.org/10.1016/j.jesp.2013.10.005.
Brumitt, J. and Cuddeford, T. (2015) ‘Current concepts of muscle and tendon adaptation to strength and conditioning.’, International journal of sports physical therapy, 10(6), pp. 748–59.
Butler, R.J., Crowell, H.P. and Davis, I.M.C. (2003) ‘Lower extremity stiffness: Implications for performance and injury’, Clinical Biomechanics, 18(6), pp. 511–517. Available at: https://doi.org/10.1016/S0268-0033(03)00071-8.
Büttner, F. et al. (2020) ‘Are questionable research practices facilitating new discoveries in sport and exercise medicine? The proportion of supported hypotheses is implausibly high’, British Journal of Sports Medicine, pp. 1–7. Available at: https://doi.org/10.1136/bjsports-2019-101863.
Button, K.S. et al. (2013) ‘Power failure: Why small sample size undermines the reliability of neuroscience’, Nature Reviews Neuroscience, 14(5), pp. 365–376. Available at: https://doi.org/10.1038/nrn3475.
Caldwell, A.R. et al. (2020) ‘Moving sport and exercise science forward: A call for the adoption of more transparent research practices’, Sports Medicine, 50(3), pp. 449–459. Available at: https://doi.org/10.1007/s40279-019-01227-1.
Caldwell, A.R. (2022) Exploring equivalence testing with the updated TOSTER R package. Available at: https://orcid.org/0000-0002-4541-6283.
Carretero-Navarro, G. et al. (2019) ‘Effect of different loading conditions on running mechanics at different velocities’, European Journal of Sport Science, 19(5), pp. 595–602. Available at: https://doi.org/10.1080/17461391.2018.1537378.
Cavagna, G.A. et al. (1988) ‘The determinants of the step frequency in running, trotting and hopping in man and other vertebrates.’, Journal of Physiology, 399, pp. 81–92. Available at: https://doi.org/https://doi.org/10.1113/jphysiol.1988.sp017069.
Cavagna, G.A., Legramandi, M.A. and Peyré-Tartaruga, L.A. (2008) ‘Old men running: Mechanical work and elastic bounce’, Proceedings of the Royal Society B: Biological Sciences, 275(1633), pp. 411–418. Available at: https://doi.org/10.1098/rspb.2007.1288.
Chambers, C.D. et al. (2014) ‘Instead of “playing the game” it is time to change the rules: Registered Reports at AIMS Neuroscience and beyond’, AIMS Neuroscience, 1(1), pp. 4–17. Available at: https://doi.org/10.3934/Neuroscience.2014.1.4.
Coleman, D.R. et al. (2012) ‘Leg stiffness in human running: Comparison of estimates derived from previously published models to direct kinematic-kinetic measures’, Journal of Biomechanics, 45(11), pp. 1987–1991. Available at: https://doi.org/10.1016/j.jbiomech.2012.05.010.
Dalleau, G. et al. (1998) ‘The spring-mass model and the energy cost of treadmill running’, European Journal of Applied Physiology and Occupational Physiology, 77(3), pp. 257–263. Available at: https://doi.org/10.1007/s004210050330.
Errington, T.M. et al. (2021) ‘Investigating the replicability of preclinical cancer biology.’, eLife, 10, pp. 1–30. Available at: https://doi.org/10.7554/eLife.71601.
Farley, C.T. et al. (1991) ‘Hopping frequency in humans: A test of how springs set stride frequency in bouncing gaits’, Journal of Applied Physiology, 71(6), pp. 2127–2132. Available at: https://doi.org/10.1152/jappl.1991.71.6.2127.
Farley, C.T. and González, O. (1996) ‘Leg stiffness and stride frequency in human running’, Journal of Biomechanics, 29(2), pp. 181–186. Available at: https://doi.org/10.1016/0021-9290(95)00029-1.
Ferris, D.P., Louie, M. and Farley, C.T. (1998) ‘Running in the real world: Adjusting leg stiffness for different surfaces’, Proceedings of the Royal Society B: Biological Sciences, 265(1400), pp. 989–994. Available at: https://doi.org/10.1098/rspb.1998.0388.
Günther, M. and Blickhan, R. (2002) ‘Joint stiffness of the ankle and the knee in running’, Journal of Biomechanics, 35(11), pp. 1459–1474. Available at: https://doi.org/10.1016/S0021-9290(02)00183-5.
Halperin, I. et al. (2018) ‘Strengthening the practice of exercise and sport-science research’, International Journal of Sports Physiology and Performance, 13(2), pp. 127–134. Available at: https://doi.org/10.1123/ijspp.2017-0322.
He, J., Kram, R. and McMahon, T.A. (1991) ‘Mechanics of Running Under Simulated Low Gravity’, Journal of Applied Physiology, 71(3), pp. 863–870. Available at: https://doi.org/10.1152/jappl.1991.71.3.863.
Heneghan, C. et al. (2012) ‘Forty years of sports performance research and little insight gained.’, BMJ, 345. Available at: https://doi.org/10.1136/bmj.e4797.
Kim, S. and Park, S. (2011) ‘Leg stiffness increases with speed to modulate gait frequency and propulsion energy’, Journal of Biomechanics, 44(7), pp. 1253–1258. Available at: https://doi.org/10.1016/j.jbiomech.2011.02.072.
Kramer, A. et al. (2012) ‘Leg stiffness can be maintained during reactive hopping despite modified acceleration conditions’, Journal of Biomechanics, 45(10), pp. 1816–1822. Available at: https://doi.org/10.1016/j.jbiomech.2012.04.014.
Kuitunen, S., Komi, P. V. and Kyröläinen, H. (2002) ‘Knee and ankle joint stiffness in sprint running’, Medicine and Science in Sports and Exercise, 34(1), pp. 166–173. Available at: https://doi.org/10.1097/00005768-200201000-00025.
Kyröläinen, H., Belli, A. and Komi, P. V. (2001) ‘Biomechanical factors affecting running economy’, Medicine and Science in Sports and Exercise, 33(8), pp. 1330–1337. Available at: https://doi.org/10.1097/00005768-200108000-00014.
Mcmahon, T.A. and Cheng, G.C. (1990) ‘The mechanics of running: How does stiffness couple with speed?’, Journal of Biomechanics, 23, pp. 65–78. Available at: https://doi.org/https://doi.org/10.1016/0021-9290(90)90042-2.
McMahon, T.A. and Cheng, G.C. (1990) ‘The mechanics of running: How does stiffness couple with speed?’, Journal of Biomechanics, 23(SUPPL. 1), pp. 65–78. Available at: https://doi.org/10.1016/0021-9290(90)90042-2.
McMahon, T.A., Valiant, G. and Frederick, E.C. (1987) ‘Groucho running’, Journal of Applied Physiology, 62(6), pp. 2326–2337. Available at: https://doi.org/10.1152/jappl.1987.62.6.2326.
Mesquida, C. et al. (2022) ‘Replication concerns in sports and exercise science: a narrative review of selected methodological issues in the field’, Royal Society Open Science, 9(220946). Available at: https://doi.org/10.1098/rsos.220946.
Morin, J.B. (2005) ‘A simple method for measuring lower limb stiffness during running’, Journal of Applied Biomechanics, 21, pp. 167–180. Available at: https://doi.org/10.1007/978-3-319-05633-3_8.
Murphy, J. et al. (2023) ‘Proposal of a Selection Protocol for Replication of Studies in Sports and Exercise Science’, Sports Medicine, 53, pp. 281–291. Available at: https://doi.org/10.1007/s40279-022-01749-1.
Nissen, S.B. et al. (2016) ‘Publication bias and the canonization of false facts’, eLife, 5. Available at: https://doi.org/10.7554/eLife.21451.
Nosek, B.A. and Errington, T.M. (2020) ‘What is replication?’, PLoS Biology, 18(3), pp. 1–8. Available at: https://doi.org/10.1371/journal.pbio.3000691.
Open Science Collaboration (2015) ‘Estimating the reproducibility of psychological science’, Science, 349(6251). Available at: https://doi.org/10.1126/science.aac4716.
Pappas, P. et al. (2014) ‘Reliabilities of leg and vertical stiffness during treadmill running’, Sports Biomechanics, 13(4), pp. 391–399. Available at: https://doi.org/10.1080/14763141.2014.981853.
Schmidt, S. (2009) ‘Shall we really do it again? The powerful concept of replication is neglected in the social sciences’, Review of General Psychology, 13(2), pp. 90–100. Available at: https://doi.org/10.1037/a0015108.
Silder, A., Besier, T. and Delp, S.L. (2015) ‘Running with a load increases leg stiffness’, Journal of Biomechanics, 48(6), pp. 1003–1008. Available at: https://doi.org/10.1016/j.jbiomech.2015.01.051.
Simons, D.J. (2014) ‘The value of direct replication’, Perspectives on Psychological Science, 9(1), pp. 76–80. Available at: https://doi.org/10.1177/1745691613514755.
Struzik, A. et al. (2021) ‘Application of leg, vertical, and joint stiffness in running performance: A literature overview’, Applied Bionics and Biomechanics, 2021. Available at: https://doi.org/10.1155/2021/9914278.
Teunissen, L.P.J., Grabowski, A. and Kram, R. (2007) ‘Effects of independently altering body weight and body mass on the metabolic cost of running’, Journal of Experimental Biology, 210(24), pp. 4418–4427. Available at: https://doi.org/10.1242/jeb.004481.